PMPCB Antibody

ProSci
Product Code: PSI-61-745
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-745-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PMPCB Antibody in mouse kidney tissue lysates (35ug/lane)
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PMPCB Antibody IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of PMPCB Antibody in mouse kidney tissue lysates (35ug/lane)
PMPCB Antibody IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Mitochondrial-processing peptidase subunit beta, Beta-MPP, P-52, PMPCB, MPPB
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
PMPCB is a member of the peptidase M16 family and encodes a protein with a zinc-binding motif. This protein is located in the mitochondrial matrix and catalyzes the cleavage of the leader peptides of precursor proteins newly imported into the mitochondria, though it only functions as part of a heterodimeric complex.
Background References:
  • Luczynski, W., et al. Neoplasma 56(5):428-434(2009)
  • Schwer, B., et al. J. Cell Biol. 158(4):647-657(2002)
  • Nagao, Y., et al. J. Biol. Chem. 275(44):34552-34556(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PMPCB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 18-46 amino acids from the N-terminal region of human PMPCB.
NCBI Gene ID #:
9512
NCBI Official Name:
Mitochondrial-processing peptidase subunit beta
NCBI Official Symbol:
PMPCB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
O75439
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle
Swissprot #:
O75439
User NOte:
Optimal dilutions for each application to be determined by the researcher.