AKT2 Antibody

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ProSci
Product Code: PSI-62-606
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-606-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of AKT2 Antibody in NCI-H460 cell line lysates (35ug/lane)
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Western blot analysis of AKT2 Antibody in mouse brain tissue lysates (35ug/lane)
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Confocal immunofluorescent analysis of AKT2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of AKT2 Antibody in NCI-H460 cell line lysates (35ug/lane)
Western blot analysis of AKT2 Antibody in mouse brain tissue lysates (35ug/lane)
Confocal immunofluorescent analysis of AKT2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, AKT2
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
AKT2 is a putative oncogenic protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.
Background References:
  • Vojtek, A.B., et al., Mol. Cell. Biol. 23(13):4417-4427 (2003). Yuan, Z.Q., et al., J. Biol. Chem. 278(26):23432-23440 (2003). Jones, D.T., et al., Blood 101(8):3174-3180 (2003). Brozinick, J.T. Jr., et al., Diabetes 52(4):935-941 (2003). Miyakawa, M., et al., Endocr. J. 50(1):77-83 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AKT2 antibody is generated from rabbits immunized with human AKT2 recombinant protein.
NCBI Gene ID #:
208
NCBI Official Name:
RAC-beta serine/threonine-protein kinase
NCBI Official Symbol:
AKT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
P31751
Protein GI Number:
1170703
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Immunology,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P31751
User NOte:
Optimal dilutions for each application to be determined by the researcher.