FUT4 Antibody

ProSci
Product Code: PSI-56-210
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-210-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
2 / 3
FUT4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
FUT4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Alpha-(1,3)-fucosyltransferase 4, 241-, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Fuc-TIV, FucT-IV, Galactoside 3-L-fucosyltransferase, FUT4, ELFT, FCT3A
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15).
Background References:
  • Yang, X.S., et al. J. Cell. Physiol. 225(2):612-619(2010) Trubiani, O., et al. J. Cell. Physiol. 225(1):123-131(2010) Pruszak, J., et al. Stem Cells 27(12):2928-2940(2009) Ogata, K., et al. Haematologica 94(8):1066-1074(2009) Read, T.A., et al. Cancer Cell 15(2):135-147(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FUT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495-524 amino acids from the C-terminal region of human FUT4.
NCBI Gene ID #:
2526
NCBI Official Name:
Alpha-(1,3)-fucosyltransferase 4
NCBI Official Symbol:
FUT4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
59 kDa
Protein Accession #:
P22083
Protein GI Number:
226694189
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Immunology,Signal Transduction
Swissprot #:
P22083
User NOte:
Optimal dilutions for each application to be determined by the researcher.