TIMP3 Antibody

Contact us
ProSci
Product Code: PSI-63-791
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-791-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis of TIMP3 Antibody in HepG2 cell line lysates (35ug/lane)
2 / 4
Western blot analysis of TIMP3 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse NIH-3T3 cell line lysate.
3 / 4
4 / 4
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
❮❯

Western blot analysis of TIMP3 Antibody in HepG2 cell line lysates (35ug/lane)
Western blot analysis of TIMP3 Antibody Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse NIH-3T3 cell line lysate.
Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Metalloproteinase inhibitor 3, Protein MIG-5, Tissue inhibitor of metalloproteinases 3, TIMP-3, TIMP3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
TIMP3 is one of inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM).
Background References:
  • Jacobson,S.G., et.al., Nat. Genet. 11 (1), 27-32 (1995) Wick,M., et.al., Biochem. J. 311 (PT 2), 549-554 (1995)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Rabbit, Rat
Immunogen:
This TIMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 113-139 amino acids from the Central region of human TIMP3.
NCBI Gene ID #:
7078
NCBI Official Name:
Metalloproteinase inhibitor 3
NCBI Official Symbol:
TIMP3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
24 kDa
Protein Accession #:
P35625
Protein GI Number:
730948
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P35625
User NOte:
Optimal dilutions for each application to be determined by the researcher.