Vimentin Antibody

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ProSci
Product Code: PSI-61-468
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-468-400ul400ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent confocal image of SY5Y cells stained with Vimentin (S82) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Vimentin (S82) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).
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Western blot analysis of Vimentin-S82 in Hela cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Fluorescent confocal image of SY5Y cells stained with Vimentin (S82) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Vimentin (S82) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the Vimentin immunosignal to the cytoskeleton, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000026025).
Western blot analysis of Vimentin-S82 in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Vimentin, VIM
Application Note:
For IF starting dilution is: 1:200

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Along with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.
Background References:
  • References for protein:
  • Whipple,R.A.,Cancer Res. 68 (14), 5678-5688 (2008)
  • Garcia-Verdugo,I.,Biochemistry 47 (18), 5127-5138 (2008)
  • Merdes,A., J. Cell Biol. 115 (2), 397-410 (1991)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Hamster, Monkey, Rat
Immunogen:
This Vimentin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-90 amino acids from human Vimentin.
NCBI Gene ID #:
7431
NCBI Official Name:
Vimentin
NCBI Official Symbol:
VIM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
P08670
Protein GI Number:
55977767
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Neuroscience,Signal Transduction
Swissprot #:
P08670
User NOte:
Optimal dilutions for each application to be determined by the researcher.