NDUFS2 Antibody

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ProSci
Product Code: PSI-64-103
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-64-103-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from A431, Hela cell line, mouse brain and rat brain tissue lysate(from left to right), using NDUFS2 Antibody at 1:1000 at each lane.
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NDUFS2 Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis of lysates from A431, Hela cell line, mouse brain and rat brain tissue lysate(from left to right), using NDUFS2 Antibody at 1:1000 at each lane.
NDUFS2 Antibody IHC analysis in formalin fixed and paraffin embedded hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial, Complex I-49kD, CI-49kD, NADH-ubiquinone oxidoreductase 49 kDa subunit, NDUFS2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
NDUFS2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (complex I). Mammalian mitochondrial complex I is composed of at least 43 different subunits, 7 of which are encoded by the mitochondrial genome, and the rest are the products of nuclear genes. The iron-sulfur protein fraction of complex I is made up of 7 subunits, including this gene product. Complex I catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria.
Background References:
  • Saada, A., et al. Am. J. Hum. Genet. 84(6):718-727(2009) Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17(12):3558-3566(2008) Starr, J.M., et al. Mech. Ageing Dev. 129(12):745-751(2008) Ban, M., et al. PLoS ONE(8), E2891 (2008) Triepels, R.H., et al. J. Biol. Chem. 276(12):8892-8897(2001)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This NDUFS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human NDUFS2.
NCBI Gene ID #:
4720
NCBI Official Name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial
NCBI Official Symbol:
NDUFS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
53 kDa
Protein Accession #:
O75306
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Obesity,Signal Transduction
Swissprot #:
O75306
User NOte:
Optimal dilutions for each application to be determined by the researcher.