Protein Kinase A regulatory subunit I beta Antibody
| Code | Size | Price |
|---|
| PSI-62-621-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
cAMP-dependent protein kinase type I-beta regulatory subunit, PRKAR1B
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
The second messenger cyclic AMP (cAMP) mediates diverse cellular
responses to external signals such as proliferation, ion transport, regulation
of metabolism and gene transcription by activation of the cAMP-dependent
protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to
the two regulatory subunits of the tetrameric PKA holoenzyme resulting in
release of active catalytic subunits. Three catalytic (C) subunits have been
identified, designated Cα, Cβ and Cγ, that each represent specific gene
products. Cα and Cβ are closely related (93% amino acid sequence similari-
ty), whereas Cγ displays 83% and 79% similarity to Cα and Cβ, respectively.
Activation of transcription upon elevation of cAMP levels results from
translocation of PKA to the nucleus where it phosphorylates the transcrip-
tion factor cAMP response element binding protein (CREB) on serine 133
which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus
linking phospho-CREB to the pol II transcription initiation complex.
Background References:
- Solberg, R., et al., Exp. Cell Res. 214(2):595-605 (1994).
- Solberg, R., et al., Biochem. Biophys. Res. Commun. 176(1):166-172 (1991).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Protein Kinase A regulatory subunit I beta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-80 amino acids from the N-terminal region of human Protein Kinase A regulatory subunit I beta.
NCBI Gene ID #:
5575
NCBI Official Name:
cAMP-dependent protein kinase type I-beta regulatory subunit
NCBI Official Symbol:
PRKAR1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
43 kDa
Protein Accession #:
P31321
Protein GI Number:
229463042
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Obesity,Signal Transduction
Swissprot #:
P31321
User NOte:
Optimal dilutions for each application to be determined by the researcher.