HRAS Antibody
| Code | Size | Price |
|---|
| PSI-55-979-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunofluorescence (IF)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase HRas, N-terminally processed, HRAS, HRAS1
Application Note:
For WB starting dilution is: 1:1000
For IF starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
Background:
This gene belongs to the Ras oncogene family, whose
members are related to the transforming genes of mammalian sarcoma
retroviruses. The products encoded by these genes function in
signal transduction pathways. These proteins can bind GTP and GDP,
and they have intrinsic GTPase activity. This protein undergoes a
continuous cycle of de- and re-palmitoylation, which regulates its
rapid exchange between the plasma membrane and the Golgi apparatus.
Mutations in this gene cause Costello syndrome, a disease
characterized by increased growth at the prenatal stage, growth
deficiency at the postnatal stage, predisposition to tumor
formation, mental retardation, skin and musculoskeletal
abnormalities, distinctive facial appearance and cardiovascular
abnormalities. Defects in this gene are implicated in a variety of
cancers, including bladder cancer, follicular thyroid cancer, and
oral squamous cell carcinoma. Multiple transcript variants, which
encode different isoforms, have been identified for this gene.
Background References:
- Ma, Z., et al. Oncogene 29(41):5559-5567(2010)
- van Engen-van Grunsven, A.C., et al. Am. J. Surg. Pathol. 34(10):1436-1441(2010)
- Li, H., et al. Oncogene 29(36):5083-5094(2010)
- Kwack, K.B., et al. KoreanGastroenterol 56(2):78-82(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Rat
Immunogen:
This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-176 amino acids from the C-terminal region of human HRAS.
NCBI Gene ID #:
3265
NCBI Official Name:
GTPase HRas
NCBI Official Symbol:
HRAS
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
21 kDa
Protein Accession #:
P01112
Protein GI Number:
131869
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P01112
User NOte:
Optimal dilutions for each application to be determined by the researcher.