Glypican 3 Antibody

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ProSci
Product Code: PSI-62-255
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-255-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunofluorecence staining of anti-GPC3 Pab on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1:50. Original magnification 1:400. Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.
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Western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).
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Immunofluorecence staining of anti-GPC3 Pab on HepG2 cells. The cells were acetone fixated. Antibody dilution of 1:50. Original magnification 1:400. Data and protocol courtesy of Dr. Mariana Dabeva, Department of Medicine at Albert Einstein College of Medicine.
Western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the GPC3 antibody detected the GPC3 protein (arrow).

Documents

Further Information

Additional Names:
Glypican-3, GTR2-2, Intestinal protein OCI-5, MXR7, Secreted glypican-3, GPC3, OCI5
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
GPC3 is a cell surface proteoglycan that bears heparan sulfate. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs, and may play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. Members of the glypican-related integral membrane proteoglycan family contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol (GPI) linkage. These proteins may play a role in the control of cell division, growth regulation, and tumor predisposition. Deletion mutations in GPC3 are the cause of Simpson-Golabi-Behmel syndrome (SGBS), also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies.
Background References:
  • Nakatsura, T., et al., Clin. Cancer Res. 10(19):6612-6621 (2004).
  • Boily, G., et al., Br. J. Cancer 90(8):1606-1611 (2004).
  • Wichert, A., et al., Oncogene 23(4):945-955 (2004).
  • Midorikawa, Y., et al., Int. J. Cancer 103(4):455-465 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Glypican 3 (GPC3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 529-560 amino acids from the C-terminal region of human Glypican 3 (GPC3).
NCBI Gene ID #:
2719
NCBI Official Name:
Glypican-3
NCBI Official Symbol:
GPC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
P51654
Protein GI Number:
1708022
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P51654
User NOte:
Optimal dilutions for each application to be determined by the researcher.