PRDX4 Antibody

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ProSci
Product Code: PSI-56-229
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-229-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
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Western blot analysis of PRDX4 using rabbit polyclonal PRDX4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX4 gene.
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PRDX4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Western blot analysis of PRDX4 using rabbit polyclonal PRDX4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX4 gene.
PRDX4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Peroxiredoxin-4, Antioxidant enzyme AOE372, AOE37-2, Peroxiredoxin IV, Prx-IV, Thioredoxin peroxidase AO372, Thioredoxin-dependent peroxide reductase A0372, PRDX4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
Background References:
  • Jamaluddin, M., et al. J. Virol. 84(18):9533-9545(2010)
  • Davila, S., et al. Genes Immun. 11(3):232-238(2010)
  • Edvardsen, H., et al. Pharmacogenomics J. (2010) In press :
  • Wang, H.Q., et al. FEBS Lett. 583(9):1511-1515(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This PRDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the Central region of human PRDX4.
NCBI Gene ID #:
10549
NCBI Official Name:
Peroxiredoxin-4
NCBI Official Symbol:
PRDX4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
Q13162
Protein GI Number:
3024727
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
Q13162
User NOte:
Optimal dilutions for each application to be determined by the researcher.