Androgen Receptor Antibody

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ProSci
Product Code: PSI-61-403
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-403-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the AR antibody detected the AR protein (arrow).
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Western blot analysis in Hela cell line lysates (35ug/lane).This demondetected the ANDR protein (arrow).
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Western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the AR antibody detected the AR protein (arrow).
Western blot analysis in Hela cell line lysates (35ug/lane).This demondetected the ANDR protein (arrow).

Documents

Further Information

Additional Names:
Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR, DHTR, NR3C4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Androgen receptor (ANDR) has 3 major functional domains: the N-terminal domain, DNA-binding domain, and an androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. The gene for this protein contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of the protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease). Mutations are also associated with complete androgen insensitivity (CAIS). PIAS1 and PIASxalpha function as SUMO-E3 ligases toward androgen receptor; sumoylation of ANDR represses androgen receptor dependent transcription.
Background References:
  • Nishida, et al. J. Biol. Chem. 277 (44), 41311-41317 (2002)
  • Sills, E.S., et al., Int. J. Mol. Med. 9(1):45-48 (2002).
  • Chavez, B., et al., J. Hum. Genet. 46(10):560-565 (2001).
  • Ahmed, S.F., et al., J. Clin. Endocrinol. Metab. 85(2):658-665 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Pig
Immunogen:
This Androgen Receptor (ANDR) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-392 amino acids from human Androgen Receptor (ANDR).
NCBI Gene ID #:
367
NCBI Official Name:
Androgen receptor
NCBI Official Symbol:
AR
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
99 kDa
Protein Accession #:
P10275
Protein GI Number:
113830
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P10275
User NOte:
Optimal dilutions for each application to be determined by the researcher.