IGF2 Antibody

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ProSci
Product Code: PSI-55-712
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-712-200ul200ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
2 / 5
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
3 / 5
4 / 5
IGF2 Antibody (Center R54) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF2 Antibody (Center R54) for immunohistochemistry.
5 / 5
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demodetected the IGF2 protein (arrow).
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Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37?C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37?C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
IGF2 Antibody (Center R54) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF2 Antibody (Center R54) for immunohistochemistry.
Western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demodetected the IGF2 protein (arrow).

Documents

Further Information

Additional Names:
Insulin-like growth factor II, IGF-II, Somatomedin-A, Insulin-like growth factor II, Insulin-like growth factor II Ala-25 Del, Preptin, IGF2
Application Note:
For FACS starting dilution is: 1:25

For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100
Background:
This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Background References:
  • Adkins, R.M., et al. Pediatr. Res. 68(5):429-434(2010)
  • Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
  • Li, J., et al. Mol. Biol. Rep. (2010) In press :
  • Hsieh, Y.Y., et al. Anticancer Res. 30(6):2203-2208(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IGF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-68 amino acids from the Central region of human IGF2.
NCBI Gene ID #:
3481
NCBI Official Name:
Insulin-like growth factor II
NCBI Official Symbol:
IGF2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
P01344
Protein GI Number:
124255
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P01344
User NOte:
Optimal dilutions for each application to be determined by the researcher.