KRT14 Antibody

ProSci
Product Code: PSI-62-420
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-420-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Western Blot at 1:2000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 3
Formalin-fixed and paraffin-embedded human breast carcinoma with KRT14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Western Blot at 1:2000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human breast carcinoma with KRT14 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Keratin, type I cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14, KRT14
Application Note:
For WB starting dilution is: 1:2000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
KRT14 is a member of the keratin family, the most diverse group of intermediate filaments. This protein, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells.
Background References:
  • Barcelos,A.C., J. Cutan. Pathol. 36 (6), 647-654 (2009) Romano,R.A., PLoS ONE(5), E5623 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Bovine
Immunogen:
This KRT14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-303 amino acids from the Central region of human KRT14.
NCBI Gene ID #:
3861
NCBI Official Name:
Keratin, type I cytoskeletal 14
NCBI Official Symbol:
KRT14
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
P02533
Protein GI Number:
229463044
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P02533
User NOte:
Optimal dilutions for each application to be determined by the researcher.