DAPK1 Antibody

ProSci
Product Code: PSI-62-737
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-737-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 3
Antibody is used in Western blot to detect DAPK1 in mouse liver tissue lysate.
2 / 3
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Antibody is used in Western blot to detect DAPK1 in mouse liver tissue lysate.
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with DAPK1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Death-associated protein kinase 1, DAP kinase 1, DAPK1, DAPK
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death (apoptosis). DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate.
Background References:
  • Shohat, G., et al., J. Biol. Chem. 276(50):47460-47467 (2001).
  • Inbal, B., et al., Mol. Cell. Biol. 20(3):1044-1054 (2000).
  • Deiss, L.P., et al., Genes Dev. 9(1):15-30 (1995).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DAPK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1360-1389 amino acids from the C-terminal region of human DAPK1.
NCBI Gene ID #:
1612
NCBI Official Name:
Death-associated protein kinase 1
NCBI Official Symbol:
DAPK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
160 kDa
Protein Accession #:
P53355
Protein GI Number:
317373595
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Cell Cycle,Signal Transduction
Swissprot #:
P53355
User NOte:
Optimal dilutions for each application to be determined by the researcher.