IL-27 Antibody

ProSci
Product Code: PSI-58-928
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-928-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of IL27 using rabbit polyclonal IL27 Antibody (C71) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL27 gene.
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IL-27 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
3 / 3

Western blot analysis of IL27 using rabbit polyclonal IL27 Antibody (C71) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the IL27 gene.
IL-27 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Interleukin-17D, IL-17D, Interleukin-27, IL-27, IL17D, IL27
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
IL-27 is a cytokine that shares the sequence similarity with IL17. The treatment of endothelial cells with this cytokine has been shown to stimulate the production of other cytokines including IL6, IL8 and CSF2/ GM-CSF. The increased expression of IL8 induced by this cytokine was found to be NF-kappa B-dependent.
Background References:
  • Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003).
  • Starnes, T., et al., J. Immunol. 169(2):642-646 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IL-27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-87 amino acids from the Central region of human IL-27.
NCBI Gene ID #:
53342
NCBI Official Name:
Interleukin-17D
NCBI Official Symbol:
IL17D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
Q8TAD2
Protein GI Number:
46576614
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Immunology
Swissprot #:
Q8TAD2
User NOte:
Optimal dilutions for each application to be determined by the researcher.