IL17B Antibody

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ProSci
Product Code: PSI-56-167
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-167-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in mouse spleen tissue lysates (35ug/lane).
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IL17B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Confocal immunofluorescent analysis of IL17B Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse spleen tissue lysates (35ug/lane).
IL17B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of IL17B Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Interleukin-17B, IL-17B, Cytokine Zcyto7, Interleukin-20, IL-20, Neuronal interleukin-17-related factor, IL17B, IL20, NIRF, ZCYTO7
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies.
Background References:
  • Koskinen, L.L., et al. Tissue Antigens 74(5):408-416(2009)
  • Wang, X., et al. Ann. Otol. Rhinol. Laryngol. 115(6):450-456(2006)
  • Ryan, A.W., et al. Tissue Antigens 65(2):150-155(2005)
  • Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This IL17B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 39-66 amino acids from the Central region of human IL17B.
NCBI Gene ID #:
27190
NCBI Official Name:
Interleukin-17B
NCBI Official Symbol:
IL17B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
Q9UHF5
Protein GI Number:
20143877
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology
Swissprot #:
Q9UHF5
User NOte:
Optimal dilutions for each application to be determined by the researcher.