MME Antibody

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ProSci
Product Code: PSI-62-829
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-829-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from Ramos cell line,mouse kidney and rat kidney tissue (from left to right),using Neprilysin Antibody .AP7329c was diluted at 1:1000 at each lane.
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Confocal immunofluorescent analysis of MME Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of lysates from Ramos cell line,mouse kidney and rat kidney tissue (from left to right),using Neprilysin Antibody .AP7329c was diluted at 1:1000 at each lane.
Confocal immunofluorescent analysis of MME Antibody with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Neprilysin, Atriopeptidase, Common acute lymphocytic leukemia antigen, CALLA, Enkephalinase, Neutral endopeptidase 2411, NEP, Neutral endopeptidase, Skin fibroblast elastase, SFE, CD10, MME, EPN
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
MME is a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a oprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
Background References:
  • Dakka,N., Bellaoui,H. Pediatr Hematol Oncol 26 (4), 216-231 (2009)
  • Wang,R., Wang,S. J. Neurochem. 108 (4), 1072-1082 (2009)
  • Shipp,M.A. Proc. Natl. Acad. Sci. U.S.A. 88 (23), 10662-10666 (1991)
  • Shipp,M.A. Proc. Natl. Acad. Sci. U.S.A. 86 (1), 297-301 (1989)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This MME antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-302 amino acids from the Central region of human MME.
NCBI Gene ID #:
4311
NCBI Official Name:
Neprilysin
NCBI Official Symbol:
MME
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
86 kDa
Protein Accession #:
P08473
Protein GI Number:
128062
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P08473
User NOte:
Optimal dilutions for each application to be determined by the researcher.