Mouse Map3k7 Antibody

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ProSci
Product Code: PSI-57-351
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-351-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
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Western blot analysis of Map3k7 using rabbit polyclonal Mouse Map3k7 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k7 gene.
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Mouse Map3k7 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis of Map3k7 using rabbit polyclonal Mouse Map3k7 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k7 gene.
Mouse Map3k7 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1, Map3k7, Tak1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.
Background References:
  • Kajino-Sakamoto, R., et al. J. Immunol. 185(8):4729-4737(2010)
  • Wu, Z.H., et al. Mol. Cell 40(1):75-86(2010)
  • Scholz, R., et al. J. Biol. Chem. 285(33):25753-25766(2010)
  • Greenblatt, M.B., et al. J. Clin. Invest. 120(7):2457-2473(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This Mouse Map3k7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-160 amino acids from the N-terminal region of mouse Map3k7.
NCBI Gene ID #:
26409
NCBI Official Name:
Mitogen-activated protein kinase kinase kinase 7
NCBI Official Symbol:
Map3k7
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
64 kDa
Protein Accession #:
Q62073
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
Q62073
User NOte:
Optimal dilutions for each application to be determined by the researcher.