Mouse Map2k3 Antibody

ProSci
Product Code: PSI-57-736
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-736-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
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Western blot analysis of Map2k3 using rabbit polyclonal Mouse Map2k3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k3 gene.
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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).

Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis of Map2k3 using rabbit polyclonal Mouse Map2k3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map2k3 gene.
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).

Further Information

Additional Names:
Dual specificity mitogen-activated protein kinase kinase 3, MAP kinase kinase 3, MAPKK 3, MAPK/ERK kinase 3, MEK 3, Map2k3, Mkk3, Prkmk3
Application Note:
For WB starting dilution is: 1:1000
Background:
Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38 (By similarity).
Background References:
  • Yang, Z., et al. J. Immunol. 185(10):6205-6213(2010)
  • Remy, G., et al. Cell. Signal. 22(4):660-667(2010)
  • Behren, A., et al. Oncogene 29(10):1519-1530(2010)
  • Nagelin, M.H., et al. J. Biol. Chem. 284(45):31303-31314(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Map2k3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-160 amino acids from the Central region of mouse Map2k3.
NCBI Gene ID #:
26397
NCBI Official Name:
Dual specificity mitogen-activated protein kinase kinase 3
NCBI Official Symbol:
Map2k3
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
39 kDa
Protein Accession #:
O09110
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Immunology,Signal Transduction,
Swissprot #:
O09110
User NOte:
Optimal dilutions for each application to be determined by the researcher.