FYN Antibody

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ProSci
Product Code: PSI-63-161
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-161-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of FYN using rabbit polyclonal FYN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FYN gene.
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Western blot analysis of anti-FYN Pab in mouse liver tissue lysate
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of FYN using rabbit polyclonal FYN Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FYN gene.
Western blot analysis of anti-FYN Pab in mouse liver tissue lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Tyrosine-protein kinase Fyn, Proto-oncogene Syn, Proto-oncogene c-Fyn, Src-like kinase, SLK, p59-Fyn, FYN
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100
Background:
FYN is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein.
Background References:
  • Taniguchi, S., et al., Biochem. Biophys. Res. Commun. 306(1):151-155 (2003). Chan, B., et al., Nat. Cell Biol. 5(2):155-160 (2003). Goldsmith, J.F., et al., Biochem. Biophys. Res. Commun. 298(4):501-504 (2002). Freund, C., et al., EMBO J. 21(22):5985-5995 (2002). Parravicini, V., et al., Nat. Immunol. 3(8):741-748 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Rat
Immunogen:
This FYN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-43 amino acids from the N-terminal region of human FYN.
NCBI Gene ID #:
2534
NCBI Official Name:
Tyrosine-protein kinase Fyn
NCBI Official Symbol:
FYN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
P06241
Protein GI Number:
125370
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
P06241
User NOte:
Optimal dilutions for each application to be determined by the researcher.