FGFR1 Antibody

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ProSci
Product Code: PSI-63-079
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-079-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Flow cytometric analysis of MCF-7 cells using FGFR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
2 / 4
Antibody is used in Western blot to detect FGFR1 in NIH-3T3 cell lysate.
3 / 4
4 / 4
Colocalization of A1B3 and FGFR1 using IF. Confluent ECs (A) or HFFs (B) were treated with or without 100 ng/mL FGF-2 in the presence or absence of 10/mL fibrinogen. After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 10/mL FGFR1 and 7E3 antibody. FGFR is visualized as red fluorescence (i,iv,vii), A1B3 is visualized as green fluorescence (ii,v,viii), and colocalization of FGF-2 and fibrinogen receptors is shown as yellow fluorescence (iii,vi,ix). Insets represent the background staining for red (i) and green (ii) fluorescence. Bars represent 25 .
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Flow cytometric analysis of MCF-7 cells using FGFR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Antibody is used in Western blot to detect FGFR1 in NIH-3T3 cell lysate.
Colocalization of A1B3 and FGFR1 using IF. Confluent ECs (A) or HFFs (B) were treated with or without 100 ng/mL FGF-2 in the presence or absence of 10/mL fibrinogen. After 1 hour, cells were washed and fixed with 3.7% formaldehyde and stained using 10/mL FGFR1 and 7E3 antibody. FGFR is visualized as red fluorescence (i,iv,vii), A1B3 is visualized as green fluorescence (ii,v,viii), and colocalization of FGF-2 and fibrinogen receptors is shown as yellow fluorescence (iii,vi,ix). Insets represent the background staining for red (i) and green (ii) fluorescence. Bars represent 25 .

Documents

Further Information

Additional Names:
Fibroblast growth factor receptor 1, FGFR-1, Basic fibroblast growth factor receptor 1, BFGFR, bFGF-R-1, Fms-like tyrosine kinase 2, FLT-2, N-sam, Proto-oncogene c-Fgr, CD331, FGFR1, BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR
Application Note:
For FACS starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:50~100
Background:
FGFR1 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene can lead to Pfeiffer syndrome and Jackson-Weiss syndrome. The genomic organization of the gene is very similar to family members 2-4, encompassing 19 exons that are subject to complex alternative splicing, which allows for structural, tissue expression and ligand affinity variations among the isoforms.
Background References:
  • Jiao, J., et al., Arch. Biochem. Biophys. 410(2):187-200 (2003).
  • Fu, L., et al., J. Comp. Neurol. 462(2):265-273 (2003).
  • Lundin, L., et al., Exp. Cell Res. 287(1):190-198 (2003).
  • Kiselyov, V.V., et al., Structure (Camb.) 11(6):691-701 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FGFR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19~48 amino acids from the N-terminal region of human FGFR1.
NCBI Gene ID #:
2260
NCBI Official Name:
Fibroblast growth factor receptor 1
NCBI Official Symbol:
FGFR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
92 kDa
Protein Accession #:
P11362
Protein GI Number:
120046
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Neuroscience,Signal Transduction
Swissprot #:
P11362
User NOte:
Optimal dilutions for each application to be determined by the researcher.