HIF1Alpha Antibody
| Code | Size | Price |
|---|
| PSI-63-185-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Fluorescence-activated cell sorting (FACS)
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Hypoxia-inducible factor 1-alpha, HIF-1-alpha, HIF1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, bHLHe78, Member of PAS protein 1, PAS domain-containing protein 8, HIF1A, BHLHE78, MOP1, PASD8
Application Note:
For WB starting dilution is: 1:250
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:50~100
For IF starting dilution is: 1:10~50
For FACS starting dilution is: 1:10~50
Background:
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.
Background References:
- Lee, M.N., et al. J. Natl. Cancer Inst. 102(6):426-442(2010) Mayer, A., et al. Adv. Exp. Med. Biol. 662, 399-405 (2010)Brouwer, E., et al. Clin. Exp. Rheumatol. 27(6):945-951(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Bovine
Immunogen:
This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the Central region of human HIF1Alpha.
NCBI Gene ID #:
3091
NCBI Official Name:
Hypoxia-inducible factor 1-alpha
NCBI Official Symbol:
HIF1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
93 kDa
Protein Accession #:
Q16665
Protein GI Number:
2498017
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity
Swissprot #:
Q16665
User NOte:
Optimal dilutions for each application to be determined by the researcher.