AMPK alpha2 Antibody
Code | Size | Price |
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PSI-62-728-400ul | 400ul | £626.00 |
Quantity:
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Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Further Information
Additional Names:
5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2
Application Note:
For WB starting dilution is: 1:1000
Background:
AMPK2 alpha2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.
Background References:
- Minokoshi, Y., et al., Nature 428(6982):569-574 (2004).
- Andersson, U., et al., J. Biol. Chem. 279(13):12005-12008 (2004).
- Browne, G.J., et al., J. Biol. Chem. 279(13):12220-12231 (2004).
- Shaw, R.J., et al., Proc. Natl. Acad. Sci. U.S.A. 101(10):3329-3335 (2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This AMPK alpha2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-507 amino acids from the C-terminal region of human AMPK alpha2.
NCBI Gene ID #:
5563
NCBI Official Name:
5'-AMP-activated protein kinase catalytic subunit alpha-2
NCBI Official Symbol:
PRKAA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
P54646
Protein GI Number:
20178276
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Neuroscience,Signal Transduction
Swissprot #:
P54646
User NOte:
Optimal dilutions for each application to be determined by the researcher.