AMY2A Antibody

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ProSci
Product Code: PSI-61-920
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-920-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in CHO cell line lysates (35ug/lane).
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Confocal immunofluorescent analysis of AMY2A Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis in CHO cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of AMY2A Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Pancreatic alpha-amylase, PA,4-alpha-D-glucan glucanohydrolase, AMY2A
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas.
Background References:
  • Endo, T., et al. Diabetes 58(3):732-737(2009)
  • Baltova, S.D. Folia Med (Plovdiv) 47(1):37-41(2005)
  • Scheil, H.G., et al. Anthropol Anz 62(4):429-434(2004)
  • Numao, S., et al. J. Biol. Chem. 279(46):48282-48291(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Pig
Immunogen:
This AMY2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 484-511 amino acids from the C-terminal region of human AMY2A.
NCBI Gene ID #:
279
NCBI Official Name:
Pancreatic alpha-amylase
NCBI Official Symbol:
AMY2A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
P04746
Protein GI Number:
113803
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P04746
User NOte:
Optimal dilutions for each application to be determined by the researcher.