PCK1 Antibody
| Code | Size | Price |
|---|
| PSI-63-393-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, PCK1, PEPCK1
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For IHC-P starting dilution is: 1:50~100
Background:
This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.
Background References:
- Berger, K. et al. PLoS ONE. 4(3): e4671 (2009).
- Dunten, P., et al., J. Mol. Biol. 316(2):257-264 (2002).
- Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
- Deloukas, P., et al., Nature 414(6866):865-871 (2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-70 amino acids from the N-terminal region of human PCK1.
NCBI Gene ID #:
5105
NCBI Official Name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
NCBI Official Symbol:
PCK1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
69 kDa
Protein Accession #:
P35558
Protein GI Number:
93138710
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P35558
User NOte:
Optimal dilutions for each application to be determined by the researcher.