PC Antibody

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ProSci
Product Code: PSI-56-463
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-463-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in mouse liver tissue lysates (35ug/lane).
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Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in mouse liver tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
Pyruvate carboxylase, mitochondrial, Pyruvic carboxylase, PCB, PC
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq].
Background References:
  • MacDonald, M.J., et al. Diabetologia 52(6):1087-1091(2009)
  • Monnot, S., et al. Hum. Mutat. 30(5):734-740(2009)
  • Wang, D., et al. Mol. Genet. Metab. 95 (1-2), 31-38 (2008) :
  • Vora, S., et al. Natl MedIndia 21(3):116-119(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-82 amino acids from the N-terminal region of human PC.
NCBI Gene ID #:
5091
NCBI Official Name:
Pyruvate carboxylase, mitochondrial
NCBI Official Symbol:
PC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
130 kDa
Protein Accession #:
P11498
Protein GI Number:
1709947
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P11498
User NOte:
Optimal dilutions for each application to be determined by the researcher.