PKC mu Antibody

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ProSci
Product Code: PSI-62-597
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-597-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-PKC mu N-term Pab in mouse kidney lysate
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of anti-PKC mu N-term Pab in mouse kidney lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.

Documents

Further Information

Additional Names:
Serine/threonine-protein kinase D1, Protein kinase C mu type, Protein kinase D, nPKC-D1, nPKC-mu, PRKD1, PKD, PKD1, PRKCM
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC mu is one of the PKC family members. The protein is a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.
Background References:
  • Rao, P.S., et al., Biochem. Biophys. Res. Commun. 310(3):1032-1038 (2003).
  • Jaggi, M., et al., Biochem. Biophys. Res. Commun. 307(2):254-260 (2003).
  • Irie, A., et al., Eur. J. Immunol. 33(6):1497-1507 (2003).
  • Storz, P., et al., J. Biol. Chem. 278(20):17969-17976 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PKC mu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the N-terminal region of human PKC mu.
NCBI Gene ID #:
5587
NCBI Official Name:
Serine/threonine-protein kinase D1
NCBI Official Symbol:
PRKD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
102 kDa
Protein Accession #:
Q15139
Protein GI Number:
209572639
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q15139
User NOte:
Optimal dilutions for each application to be determined by the researcher.