GGTLA1 Antibody

ProSci
Product Code: PSI-62-877
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-877-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of GGTLA1 antibody in Y79 cell line lysates (35ug/lane)
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Western blot analysis of GGTLA1 antibody in mouse heart tissue lysates (35ug/lane)
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Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GGTLA1 antibody in Y79 cell line lysates (35ug/lane)
Western blot analysis of GGTLA1 antibody in mouse heart tissue lysates (35ug/lane)
Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Gamma-glutamyltransferase 5, GGT 5, Gamma-glutamyl transpeptidase-related enzyme, GGT-rel, Gamma-glutamyltransferase-like activity 1, Gamma-glutamyltranspeptidase 5, Glutathione hydrolase 5, Leukotriene-C4 hydrolase, Gamma-glutamyltransferase 5 heavy chain, Gamma-glutamyltransferase 5 light chain, GGT5, GGTLA1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
This gene is a member of gamma-glutamyl transpeptidase enzymes. This enzyme consists of a heavy and a light chain, and is able to hydrolyze the gamma-glutamyl moiety of glutathione. It converts leukotriene C4 to leukotriene D4, however, it doesn't convert synthetic substrates that are commonly used to assay gamma-glutamyl transpeptidase.
Background References:
  • Heisterkamp,N., Hum. Genet. 123 (4), 321-332 (2008)
  • Enoiu,M., Free Radic. Biol. Med. 29 (9), 825-833 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GGTLA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-156 amino acids from the N-terminal region of human GGTLA1.
NCBI Gene ID #:
2687
NCBI Official Name:
Gamma-glutamyltransferase 5
NCBI Official Symbol:
GGT5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
62 kDa
Protein Accession #:
P36269
Protein GI Number:
116242493
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P36269
User NOte:
Optimal dilutions for each application to be determined by the researcher.