HMGCS1 Antibody

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ProSci
Product Code: PSI-62-382
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-62-382-400ul400ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in U87-MG cell line,mouse liver and rat liver lysates (35ug/lane).
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis in U87-MG cell line,mouse liver and rat liver lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HMGCS1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Hydroxymethylglutaryl-CoA synthase, cytoplasmic, HMG-CoA synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, HMGCS1, HMGCS
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
HMGCS1 condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
Background References:
  • Vock,C., Cell. Physiol. Biochem. 22 (5-6), 515-524 (2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Hamster, Chicken
Immunogen:
This HMGCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-317 amino acids from the Central region of human HMGCS1.
NCBI Gene ID #:
3157
NCBI Official Name:
Hydroxymethylglutaryl-CoA synthase, cytoplasmic
NCBI Official Symbol:
HMGCS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
57 kDa
Protein Accession #:
Q01581
Protein GI Number:
1708239
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
Q01581
User NOte:
Optimal dilutions for each application to be determined by the researcher.