ACADM Antibody

ProSci
Product Code: PSI-62-487
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-487-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 4
Western blot analysis of ACADM Antibody in mouse heart tissue lysates (35ug/lane)
2 / 4
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ACADM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 4
4 / 4
Confocal immunofluorescent analysis of ACADM Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of ACADM Antibody in mouse heart tissue lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ACADM Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of ACADM Antibody with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Further Information

Additional Names:
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial, MCAD, ACADM
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
ACADM is the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway.
Background References:
  • Ferreira,A.C., et.al., Genet. Mol. Res.(2), 487-493 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This ACADM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the Central region of human ACADM.
NCBI Gene ID #:
34
NCBI Official Name:
Medium-chain specific acyl-CoA dehydrogenase, mitochondrial
NCBI Official Symbol:
ACADM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
47 kDa
Protein Accession #:
P11310
Protein GI Number:
113017
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Obesity,Signal Transduction
Swissprot #:
P11310
User NOte:
Optimal dilutions for each application to be determined by the researcher.