ARHGEF7 Antibody

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ProSci
Product Code: PSI-61-358
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-358-200ul200ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.

Documents

Further Information

Additional Names:
Rho guanine nucleotide exchange factor 7, Beta-Pix, COOL-1, PAK-interacting exchange factor beta, p85, ARHGEF7, COOL1, KIAA0142, P85SPR, PAK3BP, PIXB
Application Note:
For WB starting dilution is: 1:2000
Background:
Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.
Background References:
  • Nagase T.,et al.DNA Res. 2:167-174(1995).
  • Bagrodia S.,et al.J. Biol. Chem. 273:23633-23636(1998).
  • Bechtel S.,et al.BMC Genomics 8:399-399(2007).
  • Dunham A.,et al.Nature 428:522-528(2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ARHGEF7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 654-688 amino acids from human ARHGEF7.
NCBI Gene ID #:
8874
NCBI Official Name:
Rho guanine nucleotide exchange factor 7
NCBI Official Symbol:
ARHGEF7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
90 kDa
Protein Accession #:
Q14155
Protein GI Number:
50403776
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q14155
User NOte:
Optimal dilutions for each application to be determined by the researcher.