COL18A1 Antibody

ProSci
Product Code: PSI-63-671
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-671-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of COL18A1 Antibody in mouse lung tissue lysates (35ug/lane)
2 / 2
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of COL18A1 Antibody in mouse lung tissue lysates (35ug/lane)
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Collagen alpha-1(XVIII) chain, Endostatin, COL18A1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
COL18A1 is encoding the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein.
Background References:
  • Fu,Y.,et.al., Biochemistry 48 (49), 11655-11663 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This COL18A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 237-263 amino acids from the N-terminal region of human COL18A1.
NCBI Gene ID #:
80781
NCBI Official Name:
Collagen alpha-1(XVIII) chain
NCBI Official Symbol:
COL18A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
178 kDa
Protein Accession #:
P39060
Protein GI Number:
215274264
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P39060
User NOte:
Optimal dilutions for each application to be determined by the researcher.