ITGA5 Antibody

Contact us
ProSci
Product Code: PSI-61-961
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-961-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in mouse bladder tissue lysates (15ug/lane).This demonstrates the detected ITGA5 protein (arrow).
2 / 2
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
❮❯

Western blot analysis in mouse bladder tissue lysates (15ug/lane).This demonstrates the detected ITGA5 protein (arrow).
Flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e, Integrin alpha-5 heavy chain, Integrin alpha-5 light chain, ITGA5, FNRA
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This gene encodes the integrin alpha 5 chain. Alpha chain 5 undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 to form a fibronectin receptor. In addition to adhesion, integrins are known to participate in cell-surface mediated signalling. [provided by RefSeq].
Background References:
  • Kim, S., et al. Carcinogenesis 31(4):597-606(2010)
  • Ryu, M.H., et al. Biochem. Biophys. Res. Commun. 393(1):11-15(2010)
  • Yang, Y., et al. J. Biol. Chem. 285(1):131-141(2010)
  • Lowin, T., et al. Arthritis Rheum. 60(12):3623-3632(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ITGA5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 796-822 amino acids from the C-terminal region of human ITGA5.
NCBI Gene ID #:
3678
NCBI Official Name:
Integrin alpha-5
NCBI Official Symbol:
ITGA5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
115 kDa
Protein Accession #:
P08648
Protein GI Number:
23831237
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P08648
User NOte:
Optimal dilutions for each application to be determined by the researcher.