VILIP1 Antibody

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ProSci
Product Code: PSI-58-188
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-188-200ul200ul£662.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from human brain, mouse brain, rat brain and rat eyeball tissue lysate (from left to right), using VILIP1 Antibody at 1:1000 at each lane.
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Western blot analysis of lysates from SW480 cell line, human brain, mouse brain and rat brain tissue lysate(from left to right), using VILIP1 Antibody at 1:1000 at each lane.
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Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with VILIP1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of lysates from human brain, mouse brain, rat brain and rat eyeball tissue lysate (from left to right), using VILIP1 Antibody at 1:1000 at each lane.
Western blot analysis of lysates from SW480 cell line, human brain, mouse brain and rat brain tissue lysate(from left to right), using VILIP1 Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with VILIP1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Visinin-like protein 1, VILIP, VLP-1, Hippocalcin-like protein 3, HLP3, VSNL1, VISL1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
The visinin and visinin-like peptides represent a family of calcium-binding proteins that are highly expressed in the retina. Visinin has been shown to be a cone cell-specific protein with a molecular weight of 24 kDa. Several members of the visinin family of genes have been isolated and characterized from different species. These peptides are believed to be involved in the processes of phototransduction. The recoverin gene (RCV1) is believed to be involved in the pathophysiology of retinopathy in cancer patients.
Background References:
  • Braunewell, K.H., et al., Neuropharmacology 44(6):707-715 (2003). Lin, L., et al., J. Biol. Chem. 277(44):41872-41878 (2002). Spilker, C., et al., J. Neurosci. 22(17):7331-7339 (2002). Bernstein, H.G., et al., Neuroreport 13(4):393-396 (2002). Lin, L., et al., Biochem. Biophys. Res. Commun. 296(4):827-832 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This VILIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-150 amino acids from the C-terminal region of human VILIP1.
NCBI Gene ID #:
7447
NCBI Official Name:
Visinin-like protein 1
NCBI Official Symbol:
VSNL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
22 kDa
Protein Accession #:
P62760
Protein GI Number:
51338696
Purification:
This antibody is purified through a protein G column, followed by dialysis against PBS.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P62760
User NOte:
Optimal dilutions for each application to be determined by the researcher.