PKC beta1/2 Antibody
| Code | Size | Price |
|---|
| PSI-62-591-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Applications:
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Protein kinase C beta type, PKC-B, PKC-beta, PRKCB, PKCB, PRKCB1
Application Note:
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:2000
For WB starting dilution is: 1:2000
Background:
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC beta is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. Isoform 1 uses an alternate exon at the 3' end compared to isoform 2, which includes a part of the coding region. The resulting isoform 1 has a distinct and shorter C-terminus, as compared to isoform 2.
Background References:
- Chen, D., et al., J. Biol. Chem. 279(6):4829-4839 (2004).
- Hug, B.A., et al., J. Biol. Chem. 279(2):825-830 (2004).
- Wu, H., et al., Biochem. Biophys. Res. Commun. 311(4):948-953 (2003).
- Becker, K.P., et al., J. Biol. Chem. 278(52):52747-52754 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This PKC beta1/2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 303-334 amino acids from the Central region of human PKC beta1/2.
NCBI Gene ID #:
5579
NCBI Official Name:
Protein kinase C beta type
NCBI Official Symbol:
PRKCB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
P05771
Protein GI Number:
20141488
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P05771
User NOte:
Optimal dilutions for each application to be determined by the researcher.