COL18A1 Antibody

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ProSci
Product Code: PSI-55-358
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-358-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse liver tissue lysates (35ug/lane).
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COL18A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in mouse liver tissue lysates (35ug/lane).
COL18A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Collagen alpha-1(XVIII) chain, Endostatin, COL18A1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
COL18A1 is the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. The proteolytically produced C-terminal fragment of type XVIII collagen is endostatin, a potent antiangiogenic protein. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure.
Background References:
  • Hamano, Y., et al. J. Am. Soc. Nephrol. 21(9):1445-1455(2010)
  • Roberts, K.E., et al. Gastroenterology 139(1):130-139(2010)
  • Lee, K.S., et al. Tumour Biol. 31(3):165-170(2010)
  • Faye, C., et al. Biochem. J. 427(3):467-475(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This COL18A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1311-1339 amino acids from the Central region of human COL18A1.
NCBI Gene ID #:
80781
NCBI Official Name:
Collagen alpha-1(XVIII) chain
NCBI Official Symbol:
COL18A1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
178 kDa
Protein Accession #:
P39060
Protein GI Number:
215274264
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
P39060
User NOte:
Optimal dilutions for each application to be determined by the researcher.