PIM3 Antibody

ProSci
Product Code: PSI-62-703
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-703-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect PIM3 in mouse kidney tissue lysate.
2 / 3
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIM3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
3 / 3

Antibody is used in Western blot to detect PIM3 in mouse kidney tissue lysate.
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIM3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Serine/threonine-protein kinase pim-3, PIM3
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
PIM3 is a memberof the Ser/Thr protein kinase family that may be involved in cell cycle progression and anti-apoptosis process. This protein has been implicated in proliferation of human hepatoma cell lines. PIM3 is widely expressed, with the exception of no expression observed in colon, thymus, and small intestine. PIM3 is expressed in human hepatoma cell lines but not in normal liver tissues.
Background References:
  • Mikkers, H., et al., Mol. Cell. Biol. 24(13):6104-6115 (2004). Eichmann, A., et al., Oncogene 19(9):1215-1224 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This PIM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-288 amino acids from the C-terminal region of human PIM3.
NCBI Gene ID #:
415116
NCBI Official Name:
Serine/threonine-protein kinase pim-3
NCBI Official Symbol:
PIM3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
36 kDa
Protein Accession #:
Q86V86
Protein GI Number:
215274221
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q86V86
User NOte:
Optimal dilutions for each application to be determined by the researcher.