ERK4 Antibody

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ProSci
Product Code: PSI-62-954
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-954-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-ERK4 Pab in mouse brain tissue lysate
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ERK4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of anti-ERK4 Pab in mouse brain tissue lysate
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ERK4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Mitogen-activated protein kinase 4, MAP kinase 4, MAPK 4, Extracellular signal-regulated kinase 4, ERK-4, MAP kinase isoform p63, p63-MAPK, MAPK4, ERK4, PRKM4
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The CMGC group consists of 60 kinases including the cyclin-dependent kinase (CDK) and close relatives family, the MAP kinase (ERK) family, the glycogen synthase kinase 3 (GSK3) family, and the Cdc2-like kinase (CLK) family.
Background References:
  • Robinson, M.J., et al., Curr. Opin. Cell Biol. 9(2):180-186 (1997).
  • Davis, R.J., Mol. Reprod. Dev. 42(4):459-467 (1995).
  • Seger, R., et al., FASEB J. 9(9):726-735 (1995).
  • Li, L., et al., Oncogene 9(2):647-649 (1994).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ERK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 527-557 amino acids from the C-terminal region of human ERK4.
NCBI Gene ID #:
5596
NCBI Official Name:
Mitogen-activated protein kinase 4
NCBI Official Symbol:
MAPK4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
P31152
Protein GI Number:
215274102
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
P31152
User NOte:
Optimal dilutions for each application to be determined by the researcher.