Mouse Akt2 Antibody

ProSci
Product Code: PSI-57-345
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-57-345-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
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Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).

Further Information

Additional Names:
RAC-beta serine/threonine-protein kinase, Protein kinase Akt-2, Protein kinase B beta, PKB beta, RAC-PK-beta, Akt2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
General protein kinase capable of phosphorylating several known proteins.
Background References:
  • Zhou, F., et al. Am. J. Pathol. 177(4):2124-2133(2010) Mouton, V., et al. Biochem. J. 431(2):267-275(2010) Lazorchak, A.S., et al. Mol. Cell 39(3):433-443(2010) Goncalves, M.D., et al. PLoS ONE(9), E12707 (2010)Rotte, A., et al. Cell. Physiol. Biochem. 25(6):695-704(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This Mouse Akt2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-122 amino acids from the N-terminal region of mouse Akt2.
NCBI Gene ID #:
11652
NCBI Official Name:
RAC-beta serine/threonine-protein kinase
NCBI Official Symbol:
Akt2
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
56 kDa
Protein Accession #:
Q60823
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q60823
User NOte:
Optimal dilutions for each application to be determined by the researcher.