ZDHC2 Antibody

ProSci
Product Code: PSI-61-982
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-982-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse Neuro-2a cell line lysates (15ug/lane).
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ZDHC2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in mouse Neuro-2a cell line lysates (15ug/lane).
ZDHC2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Palmitoyltransferase ZDHHC2, Reduced expression associated with metastasis protein, Ream, Reduced expression in cancer protein, Rec, Zinc finger DHHC domain-containing protein 2, DHHC-2, Zinc finger protein 372, ZDHHC2, REAM, REC, ZNF372
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Palmitoyltransferase specific for GAP43 and DLG4/PSD95 (By similarity).
Background References:
  • Planey, S.L., et al. Mol. Biol. Cell 20(5):1454-1463(2009)
  • Sharma, C., et al. Mol. Biol. Cell 19(8):3415-3425(2008)
  • Zhang, J., et al. Mol. Cell Proteomics 7(7):1378-1388(2008)
  • Li, B., et al. J. Biol. Chem. 277(32):28870-28876(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This ZDHC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the N-terminal region of human ZDHC2.
NCBI Gene ID #:
51201
NCBI Official Name:
Palmitoyltransferase ZDHHC2
NCBI Official Symbol:
ZDHHC2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q9UIJ5
Protein GI Number:
28202111
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cancer,Signal Transduction
Swissprot #:
Q9UIJ5
User NOte:
Optimal dilutions for each application to be determined by the researcher.