MMP12 Antibody

Product Code: PSI-62-169

Supplier: ProSci

Code	Size	Price

PSI-62-169-400ul

400ul

£626.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: Rabbit Ig

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Target Species:

Human
Mouse

Applications:

Immunofluorescence (IF)
Immunohistochemistry- Paraffin Embedded (IHC-P)
Western Blot (WB)

Storage:

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody staining MMP12 in human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

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Western Blot at 1:2000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.

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Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in 293 cell line lysate.

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Western blot analysis of anti-hMMP12-R406 Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse kidney tissue lysates.

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Confocal immunofluorescent analysis of MMP12 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

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Documents

Further Information

Additional Names:

Macrophage metalloelastase, MME, Macrophage elastase, ME, hME, Matrix metalloproteinase-12, MMP-12, MMP12, HME

Application Note:

For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

Background:

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP12 may be involved in tissue injury and remodeling. This protein has significant elastolytic activity. MMP12 can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, wtih small hydrophobic residues (prefereably alanine) occupying P3. The protein is found in alveolar macrophages but not in peripheral blood monocytes. MMP12 can be induced by exposure to lypopolysaccharide, and is inhibited by dexamethasone.

Background References:

Nar, H., et al., J. Mol. Biol. 312(4):743-751 (2001).
Lang, R., et al., J. Mol. Biol. 312(4):731-742 (2001).
Gronski, T.J. Jr., et al., J. Biol. Chem. 272(18):12189-12194 (1997).
Shapiro, S.D., et al., J. Biol. Chem. 268(32):23824-23829 (1993).

Buffer:

Supplied in PBS with 0.09% (W/V) sodium azide.

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

This MMP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 391-420 amino acids from the C-terminal region of human MMP12.

NCBI Gene ID #:

4321

NCBI Official Name:

Macrophage metalloelastase

NCBI Official Symbol:

MMP12

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

54 kDa

Protein Accession #:

P39900

Protein GI Number:

729179

Purification:

This antibody is purified through a protein A column, followed by peptide affinity purification.

Research Area:

Cancer,Signal Transduction

Swissprot #:

P39900

User NOte:

Optimal dilutions for each application to be determined by the researcher.