PRKCA Antibody

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ProSci
Product Code: PSI-63-524
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-524-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of PRKCA Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRKCA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Confocal immunofluorescent analysis of PRKCA Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of PRKCA Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRKCA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of PRKCA Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Protein kinase C alpha type, PKC-A, PKC-alpha, PRKCA, PKCA, PRKACA
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50
Background:
PRKCA is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. It may play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Background References:
  • Gysin,S. and Imber,R. et.al., Eur. J. Biochem. 240 (3), 747-750 (1996) Rider,M.H., et.al., Biochem. J. 285 (PT 2), 405-411 (1992)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rabbit, Rat
Immunogen:
This PRKCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 75-103 amino acids from the N-terminal region of human PRKCA.
NCBI Gene ID #:
5578
NCBI Official Name:
Protein kinase C alpha type
NCBI Official Symbol:
PRKCA
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
P17252
Protein GI Number:
317373571
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cancer,Signal Transduction
Swissprot #:
P17252
User NOte:
Optimal dilutions for each application to be determined by the researcher.