NISCH Antibody
| Code | Size | Price |
|---|
| PSI-61-350-200ul | 200ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
- Human
- Mouse
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Nischarin, Imidazoline receptor 1, I-1, IR1, Imidazoline receptor antisera-selected protein, hIRAS, Imidazoline-1 receptor, I1R, Imidazoline-1 receptor candidate protein, I-1 receptor candidate protein, I1R candidate protein, NISCH, IRAS, KIAA0975
Application Note:
For WB starting dilution is: 1:2000
Background:
Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways (By similarity). Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (By similarity). Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation (By similarity). Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation (By similarity). Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells (By similarity). Inhibits lamellipodia formation, when overexpressed (By similarity). Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures.
Background References:
- Piletz J.E.,et al.DNA Cell Biol. 19:319-329(2000).
- Piletz J.E.,et al.Ann. N. Y. Acad. Sci. 1009:419-426(2003).
- Nagase T.,et al.DNA Res. 6:63-70(1999).
- Ota T.,et al.Nat. Genet. 36:40-45(2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NISCH antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 5-38 amino acids from human NISCH.
NCBI Gene ID #:
11188
NCBI Official Name:
Nischarin
NCBI Official Symbol:
NISCH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
167 kDa
Protein Accession #:
Q9Y2I1
Protein GI Number:
296439287
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9Y2I1
User NOte:
Optimal dilutions for each application to be determined by the researcher.