FBXO2 Antibody

ProSci
Product Code: PSI-55-088
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-088-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
2 / 2
Western blot analysis in mouse brain tissue and HepG2,293 cell line lysates (35ug/lane).

Flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in mouse brain tissue and HepG2,293 cell line lysates (35ug/lane).

Further Information

Additional Names:
F-box only protein 2, FBXO2, FBX2
Application Note:
For FACS starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. This protein is highly similar to the rat NFB42 (neural F Box 42 kDa) protein which is enriched in the nervous system and may play a role in maintaining neurons in a postmitotic state.
Background References:
  • Eom, C.Y., et al. Proc. Natl. Acad. Sci. U.S.A. 100(17):9803-9807(2003) Ilyin, G.P., et al. Gene 296 (1-2), 11-20 (2002)Yoshida, Y., et al. Nature 418(6896):438-442(2002) Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999) Cenciarelli, C., et al. Curr. Biol. 9(20):1177-1179(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FBXO2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-271 amino acids from the C-terminal region of human FBXO2.
NCBI Gene ID #:
26232
NCBI Official Name:
F-box only protein 2
NCBI Official Symbol:
FBXO2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
33 kDa
Protein Accession #:
Q9UK22
Protein GI Number:
51338836
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9UK22
User NOte:
Optimal dilutions for each application to be determined by the researcher.