FBXO9 Antibody

ProSci
Product Code: PSI-61-975
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-975-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in mouse liver tissue lysates (15ug/lane).This demonstrates the detected FBXO9 protein (arrow).
2 / 2
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in mouse liver tissue lysates (15ug/lane).This demonstrates the detected FBXO9 protein (arrow).
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
F-box only protein 9, Cross-immune reaction antigen 1, Renal carcinoma antigen NY-REN-57, FBXO9, FBX9, VCIA1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates at least 3 transcript variants diverging at the 5' terminus.
Background References:
  • Jin, J., et al. Genes Dev. 18(21):2573-2580(2004)
  • Mungall, A.J., et al. Nature 425(6960):805-811(2003)
  • Scanlan, M.J., et al. Int. J. Cancer 83(4):456-464(1999)
  • Winston, J.T., et al. Curr. Biol. 9(20):1180-1182(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This FBXO9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-373 amino acids from the C-terminal region of human FBXO9.
NCBI Gene ID #:
26268
NCBI Official Name:
F-box only protein 9
NCBI Official Symbol:
FBXO9
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
52 kDa
Protein Accession #:
Q9UK97
Protein GI Number:
13124238
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9UK97
User NOte:
Optimal dilutions for each application to be determined by the researcher.