BTG1 Antibody

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ProSci
Product Code: PSI-61-795
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-795-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
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(LEFT)Western blot analysis of BTG1 Antibody in MCF-7 cell line lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of BTG1 Antibody in mouse NIH-3T3 tissue lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.
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Western Blot at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.
(LEFT)Western blot analysis of BTG1 Antibody in MCF-7 cell line lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.(RIGHT)Western blot analysis of BTG1 Antibody in mouse NIH-3T3 tissue lysates (35ug/lane).BTG1 (arrow) was detected using the purified Pab.

Documents

Further Information

Additional Names:
Protein BTG1, B-cell translocation gene 1 protein, BTG1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
BTG1 is a member of an anti-proliferative gene family that regulates cell growth and differentiation. Expression of this gene is highest in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. The encoded protein interacts with several nuclear receptors, and functions as a coactivator of cell differentiation. This locus has been shown to be involved in a t(8;12)(q24;q22) chromosomal translocation in a case of B-cell chronic lymphocytic leukemia.
Background References:
  • Camina-Tato, M., et al. J. Neuroimmunol. 213 (1-2), 142-147 (2009) Zemunik, T., et al. Croat. Med. J. 50(1):23-33(2009) Takahashi, N., et al. Exp. Hematol. 36(12):1760-1770(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Rat
Immunogen:
This BTG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-118 amino acids from the Central region of human BTG1.
NCBI Gene ID #:
694
NCBI Official Name:
Protein BTG1
NCBI Official Symbol:
BTG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
P62324
Protein GI Number:
59799172
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
P62324
User NOte:
Optimal dilutions for each application to be determined by the researcher.