UCH37 Antibody

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ProSci
Product Code: PSI-61-090
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-090-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Antibody is used in Western blot to detect UCHL5 in mouse kidney tissue lysate.
2 / 2
HE staining of frozen human ovarian cancer tissue reacted with the primary antibody at a 1:250 dilution. Levels using the antibody on frozen tissue array (A )correlated well with the mRNA expression levels detected by Agilent expression microarray (B). 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles.
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Antibody is used in Western blot to detect UCHL5 in mouse kidney tissue lysate.
HE staining of frozen human ovarian cancer tissue reacted with the primary antibody at a 1:250 dilution. Levels using the antibody on frozen tissue array (A )correlated well with the mRNA expression levels detected by Agilent expression microarray (B). 60X magnification. Data courtesy of Marlena Fejzo, University of California, Los Angeles.

Documents

Further Information

Additional Names:
Ubiquitin carboxyl-terminal hydrolase isozyme L5, UCH-L5, Ubiquitin C-terminal hydrolase UCH37, Ubiquitin thioesterase L5, UCHL5, UCH37
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Covalent attachment of the C-terminus of ubiquitin to cellular proteins plays a role in a variety of cellular processes. Ubiquitin C-terminal hydrolysis is catalyzed by deubiquitinating (DUB) enzymes and is necessary for several functions, including liberation of monomeric ubiquitin from the precursors encoded by ubiquitin genes and recycling of ubiquitin monomers. There are 2 distinct families of DUBs, ubiquitin-specific proteases (UBPs) and ubiquitin C-terminal hydrolases (UCHs). Mayer and Wilkinson (1989) identified 4 distinct UCH activities from bovine thymus. All 4 were thiol proteases and had high-affinity binding sites for ubiquitin. Wilkinson et al. (1989) purified the predominant isozyme, UCHL3, and raised antibodies against it. By screening a human B-cell expression library with the antibodies, the authors isolated cDNAs encoding human UCHL3. Sequence comparisons revealed that the sequence of the predicted 230-amino acid human UCHL3 protein is 54% identical to that of UCHL1.
Background References:
  • M-P. et al. Proteomics. July; 9(13): 3609622(2009).
  • Hu, R.M., et al., Proc. Natl. Acad. Sci. U.S.A. 97(17):9543-9548 (2000).
  • Lai, C.H., et al., Genome Res. 10(5):703-713 (2000).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig
Immunogen:
This UCH37 (UCHL5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-87 amino acids from the N-terminal region of human UCH37 (UCHL5).
NCBI Gene ID #:
51377
NCBI Official Name:
Ubiquitin carboxyl-terminal hydrolase isozyme L5
NCBI Official Symbol:
UCHL5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
38 kDa
Protein Accession #:
Q9Y5K5
Protein GI Number:
108936023
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
Q9Y5K5
User NOte:
Optimal dilutions for each application to be determined by the researcher.