TSPAN3 Antibody

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ProSci
Product Code: PSI-61-974
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-974-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the detected TSPAN3 protein (arrow).
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TSPAN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in mouse kidney tissue lysates (15ug/lane).This demonstrates the detected TSPAN3 protein (arrow).
TSPAN3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Tetraspanin-3, Tspan-3, Tetraspanin TM4-A, Transmembrane 4 superfamily member 8, TSPAN3, TM4SF8
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The use of alternate polyadenylation sites has been found for this gene.
Background References:
  • Berditchevski, F. J. Cell. Sci. 114 (PT 23), 4143-4151 (2001) :
  • Todd, S.C., et al. Biochim. Biophys. Acta 1399(1):101-104(1998)
  • Wu, Y.J., et al. J. Biol. Chem. 266(26):17566-17572(1991)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This TSPAN3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-117 amino acids from the Central region of human TSPAN3.
NCBI Gene ID #:
10099
NCBI Official Name:
Tetraspanin-3
NCBI Official Symbol:
TSPAN3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
O60637
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
O60637
User NOte:
Optimal dilutions for each application to be determined by the researcher.