CDCA3 Antibody

ProSci
Product Code: PSI-62-360
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-360-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CDCA3 antibody in mouse spleen tissue lysates(35ug/lane)
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Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of CDCA3 antibody in mouse spleen tissue lysates(35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDCA3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Cell division cycle-associated protein 3, Gene-rich cluster protein C8, Trigger of mitotic entry protein 1, TOME-1, CDCA3, C8, GRCC8, TOME1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
CDCA3 is a F-box-like protein which is required for entry into mitosis. It acts by participating to E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at G2/M phase.
Background References:
  • Daub H., Mol. Cell 31:438-448(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CDCA3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-69 amino acids from the N-terminal region of human CDCA3.
NCBI Gene ID #:
83461
NCBI Official Name:
Cell division cycle-associated protein 3
NCBI Official Symbol:
CDCA3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
Q99618
Protein GI Number:
74732787
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Cell Cycle
Swissprot #:
Q99618
User NOte:
Optimal dilutions for each application to be determined by the researcher.