Ufc1 Antibody

ProSci
Product Code: PSI-61-140
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-140-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in mouse kidney tissue lysates (35ug/lane).
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Western blot analysis of Ufc1 using rabbit polyclonal Ufc1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Ufc1 gene.
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4 / 4
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in mouse kidney tissue lysates (35ug/lane).
Western blot analysis of Ufc1 using rabbit polyclonal Ufc1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Ufc1 gene.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Ubiquitin-fold modifier-conjugating enzyme 1, Ufm1-conjugating enzyme 1, UFC1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
Various ubiquitin-like (UBL) post-translational modifiers contribute critically to the protein degradation pathway . These UBLs are covalently linked to most target proteins through an enzymatic cascade analogous to ubiquitylation, consisting of E1 (activating), E2 (conjugating), and E3 (ligating) enzymes. Ufc1 Belongs to the ubiquitin-conjugating enzyme family, and is an E2-like enzyme which forms an intermediate with UFM1, a novel ubiquitin-fold modifier, via thioester linkage.
Background References:
  • Komatsu, M., et al., EMBO J. 23(9):1977-1986 (2004).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Ufc1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Ufc1.
NCBI Gene ID #:
51506
NCBI Official Name:
Ubiquitin-fold modifier-conjugating enzyme 1
NCBI Official Symbol:
UFC1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
19 kDa
Protein Accession #:
Q9Y3C8
Protein GI Number:
116242840
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Cell Cycle
Swissprot #:
Q9Y3C8
User NOte:
Optimal dilutions for each application to be determined by the researcher.